For corresponding page on Binding of Isaac: Rebirth Wiki, see
Bosses are strong monsters found in boss rooms at the end of a floor. The boss at the end of the floor must be defeated in order to advance to the next floor. The boss for a particular floor is chosen from the pool of bosses for the environment of the floor. Upon defeat, most bosses will drop an item from the boss item pool.
Bosses [ ]
Protected by some little buddies, the peeping uses a variety of blood barrages to attack isaac.
Fights using eyeballs.
An invulnerable boss with only one weak spot.
Fights with magic runes that change how the player fights.
Attacks the player using deadly blood lasers, and tricky projectile patterns.
Shoots the player along some platforms.
A queen bee who attacks using shockwaves and by spawning flies to fight for him.
An early boss that attacks by firing a laser towards isaac, and by leaping towards him.
A large boss that fights by summoning poopy friends.
A large blister that summons not one, not three, but TWO little balls as part of their attack!
A big portal that summons other bosses to fight for them.
This old Monstro protect himself with Ministros.
A demonic boss that fights using their vomit.